Date du document : 5 mai 2015
Dernière mise à jour le : 25 avril 2017
Education / Graduation / Career
- 1995 - Université Claude Bernard Lyon I - M2 Metabolism Endocrninology Nutrition
- 1999 - Université Claude Bernard Lyon I - PhD in Reproduction Biology
- 2000 - 2003 - Saint Jude Children's Research Hospital, Memphis,TN, USA - Post-Doctoral fellow
- 2004 - 2006 - INSERM U407 Lyon - Post-Doctoral fellow
- 2006 - 2007 - Cancer Epigenetics laboratory, CEA Saclay - Post-Doctoral fellow funded by ANR
- 2008 - 2011 - Epigenetics and Cell signaling, Institut Albert Bonniot, Grenoble - Contrat jeune chercheur Inserm
Teaching: The main disciplines
- Cellular and Molecular Biology
- Male genome reprogramming during mouse spermatogenesis
- Chromatin regulators and mouse stem cells
- The impact of testicular chromatin actors on cancer cells
Barral S, Morozumi Y, Tanaka H, Montellier E, Govin J, de Dieuleveult M, Charbonnier G, Couté Y, Puthier D, Buchou T, Boussouar F, Urahama T, Fenaille F, Curtet S, Héry P, Fernandez-Nunez N, Shiota H, Gérard M, Rousseaux S, Kurumizaka H, Khochbin S. Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells. Mol Cell. 2017 Mar 18. pii: S1097-2765(17) 30162-4. doi: 10.1016/j.molcel.2017.02.025.
- de Dieuleveult M, Yen K, Hmitou I, Depaux A, Boussouar F*, Bou Dargham D, Jounier S, Humbertclaude H, Ribierre F, Baulard C, Farrell NP, Park B, Keime C, Carrière L, Berlivet S, Gut M, Gut I, Werner M, Deleuze JF, Olaso R, Aude JC, Chantalat S, Pugh BF, Gérard M. Genome-wide nucleosome specificity and function of chromatin remodellers in ES cells. (* Second author) Nature. 2016 Feb 4;530 (7588):113-6. doi: 10.1038/nature16505. Epub 2016 Jan 27.
- Morozumi Y*, Boussouar F*, Tan M, Chaikuad A, Jamshidikia M, Colak G, He H, Nie L, Petosa C, de Dieuleveult M, Curtet S, Vitte AL, Rabatel C, Debernardi A, Cosset FL, Verhoeyen E, Emadali A, Schweifer N, Gianni D, Gut M, Guardiola P, Rousseaux S, Gérard M, Knapp S, Zhao Y, Khochbin S. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells. (* Co-first author). J Mol Cell Biol. 2016 Aug;8(4):349-62. doi: 10.1093/jmcb/mjv060.
- Cattaneo M, Morozumi Y, Perazza D, Boussouar F, Jamshidikia M, Rousseaux S, Verdel A, Khochbin S. Lessons from yeast on emerging roles of the ATAD2 protein family in gene regulation and genome organization. Mol Cells. 2014 Dec 31;37(12):851-6. doi: 10.14348/molcells.2014.0258. Epub 2014 Nov 5. Review.
- Boussouar F*, Goudarzi A, Buchou T, Shiota H, Barral S, Debernardi A, Guardiola P, Brindle PK, Martinez G, Arnoult C and Khochbin S*, Rousseaux S*. (2014) A specific CBP/p300-dependent gene expression programme drives the metabolic remodelling in late stages of spermatogenesis. Andrology 2 : 351–59. (*Co-senior authors).
- Montellier E, Boussouar F, Rousseaux S, Zhang K, Buchou T, Fenaille F, Shiota H, Debernardi A, Héry P, Curtet S, Jamshidikia M, Barral S, Holota H, Bergon A, Lopez F, Guardiola P, Pernet K, Imbert J, Petosa C, Tan M, Zhao Y, Gérard M and Khochbin S. (2013) Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B. Genes Dev. 27 (15): 1680-92.
- Gaucher J, Boussouar F, Montellier E, Curtet S, Buchou T, Bertrand S, Hery P, Jounier S, Depaux A, Vitte AL, Guardiola P, Pernet K, Debernardi A, Lopez F, Holota H, Imbert J, Wolgemuth V, Gérard M, Rousseaux S and Khochbin S. (2012) Bromodomain-dependent stage-specific male genome programming by Brdt. EMBO J 31 (19): 3809-20.
- Boussouar F, Kasper LH, Boyd K, Xu W, Biesen M, Rehg J, Baudino TA, Cleveland JL and Brindle PK (2005) Two trans-activation mechanisms cooperate for the bulk of HIF-1-responsive gene expression. EMBO J. 24: 3846-58. (*co-first authors)
- Koo SH, Flechner L, Qi L, Zhang X, Screaton, RA, Jeffries S, Hedrick S, Xu W, Boussouar F, Brindle P, Takemori H and Montminy M. (2005) The CREB Coactivator TORC2 is a Key Regulator of Fasting Glucose Metabolism. Nature 437: 1109-11.
- Kang-Decker N, Tong C, Boussouar F, Baker DJ, Xu W, Leontovich AA, Taylor WR, Brindle PK and Van Deursen JM. (2004) Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency. Cancer Cell 5 : 177-189.
- Boussouar F, Jamshidikia M, Morozumi Y, Rousseaux S and Khochbin S. (2013) Malignant genome reprogramming by ATAD2 Biochim Biophys Acta 1829 : 1010-14.
- Boussouar F, Rousseaux S and Khochbin S. (2008). A new insight into male genome reprogramming by histone variants and histone code. Cell Cycle 7: 22 : 3499-3502.
- Boussouar F* and Benahmed M. Lactate and energy metabolism in male germ cells. Trends Endocrinol Metab (2004) 15: 345-50. (* Senior author)
- Boussouar F, Grataroli R, Ji J and Benahmed M. (1999) Tumor necrosis factor-a stimulates lactate dehydrogenase-A mRNA in porcine cultured Sertoli cells: Mechanisms of action. Endocrinology 140: 3054-3062.
Interests, Projects, Ideas
The post-meiotic male genome programming is a fundamental process still poorly known. It is associated with a complete reorganization of the genome, since the majority of histones are replaced by other proteins that specifically bind to the male genome allowing its compaction it in the sperm that are protamines. Although this is a phenomenon of great importance in particular to ensure the integrity of the male genome during fertilization and its transmission to the embryo, we know virtually nothing about the fundamental mechanisms that direct it. Our work focuses on the identification and functional characterization of the factors involved in this process. It is generally accepted that the removal of histones and their replacement by transition proteins and protamines is preceded by a wave of hyperacetylation of chromatin in germ cells early in their elongation. In our laboratory, the in silico research for bromodomain-containing factors whose expression is exclusively testicular and capable of interacting specifically with lysine acetylated histones, identified two proteins; the first Brdt a double bromodomain factor, and the second, Atad2, a factor having a bromodomain and a AAA-ATPase domain. The discovery of these factors has significance not only for fertility but also for the understanding of tumorigenesis. Indeed, although the expression of these factors is testis-specific, it is greatly increased in several types of cancer. They belong to the family of "Cancer Testis Antigens". This has a double significance; they could be used as biomarkers for the diagnosis of patients and at the same time they are therapeutic targets of great importance thanks to their bromodomain and the ATPase domain. We are also interested in deciphering the role of histone variants as well as the recently discovered post-translational modifications of histones in the male genome progamming of germ cells and the impact of the deregulation of their expression in cancer.